Study on the Quality Standard of Flemingia philippinensis / 中国药房

OBJECTIVE:To establish the quality standard of Flemingia philippinensis. METHODS:The properties and micro-scopic characteristics were observed;TLC was adopted for qualitative identification of genistin and genistein;the moisture,total ash and extract were detected;HPLC was adopted for contents determination of genistin and genistein:the column was Thermo BE-TASIL C18 with mobile phase of Acetonitrile-0.5% glacial acetic acid(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 261 nm,column temperature was 25℃,and the injection volume was 10μl. RESULTS:The properties and micro-scopic characteristics of F. philippinensis and F. macrophylla showed strong specificity. TLC spots of genistin and genistein we- re clear and well separated,with no interference in negative control. The moisture was 3.69%-8.37%,total ash was 1.72%-6.74%,and extract was 5.89%-19.65%. The linear range was 0.012 9-2.588 μg for genistin(r=0.999 9)and 0.004 6-0.923 2 μg for genistein (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recoveries were 99.63%-101.87%(RSD=0.82%,n=6)and 97.19%-100.34%(RSD=1.23%,n=6). CONCLUSIONS:The method is simple,accurate and specif-ic,and can be used for the quality control of F. philippinensis.

Clinical effect on the treatment of the low-middle frequency sudden hearing loss with postaurical injection of methylprednisolone / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the clinical efficacy on the treatment of the low-middle frequency sudden hearing loss with postaurical injection of methylprednisolone.@*METHOD@#The 80 cases of the low-middle frequency sudden hearing loss were randomly divided into postaurical injection and oral hormone groups. The postaurical injection group (42 cases) received the postaurical injection of methylprednisolone, 40 mg/2 d, combined with the treatment of Ginkgo dipyidamolum and Alprostadil for 14 d; The oral hormone group (38 cases) received the oral prednisone, 1 mg/kg/d, administrated once on the morning for 3 d, if effective, prolonging for another 2 d, as mentioned above for Ginkgo dipyidamolum and Alprostadil.@*RESULT@#The total effective rate was 88.10% in postaurical injection group and 86. 4o%in oral hormone group. There was no significant difference between the twbogroups( P> 0. 5).@*CONCLUSION@#Postaurical injection of methylprednisolone for the low-middle frequency sudden hearing loss is effective, safe and simple, which may be an alternative for systemic administration of gulcocorticoid.

Analyzing Main Problems and Measurements in Financial Acceptance of Scientific Research Tasks / 中国卫生经济

There are many existing problems of scientific research task financial acceptance in policy reference, acceptance content, organization form and methods. Specific measurements and suggestions of improvement are proposed on the basis of detailed list and analysis.

Analysis on Financial Management of Scientific Research Project / 中国卫生经济

Problems of budget enforcement, financial accounting, expenditure balance and equipment material calculation exist in financial management of scientific research project , and relevant policies and suggestions are provided on this basis.

Main Problems in Research Projects Budgeting Preparation and Countermeasures / 中国卫生经济

In the current research project budgeting preparovtion, there are many problems in the arrangement of construction, literal statement, and table settings; and the description of subject expenditure’s relevant to the objectives, consistent with policies and economic rationality. Based on the detailed presentation of problems and serious analysis, strategies and suggestions on budget improvement are proposed.

Evaluation the curative effect of supracricoid partial laryngectomy for laryngeal cancer / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To analyze the oncological outcomes, functional outcomes in patients undergoing supracricoid partial laryngectomy (SCPL). Provide clinical experience for application of SCPL.@*METHOD@#A retrospective analysis of the 115 cases with laryngeal carcinoma accepted SCPL in our department from Jan 1996 to Dec 2004. Use the Kaplan-Meier method to analyze the patients'survival rate. Evaluate the value of reserve larynx function.@*RESULT@#The 5-years survival rates and the decannulation rate was 80.8%, 99.1% respectively; and the average decannulation time was 22.25 days. The mean time of removal of gastric tube was 9.57 days. The function of CHEP was superior to CHP. The vocal function of 115 cases were all achieved in general communication.@*CONCLUSION@#SCPL get better oncological and functional outcomes and allows the preservation of the basic function of the larynx. It's a safe, effective technique and deserved to generalization.

Manifestations and management of malignant lymphoma in head and neck / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the clinical feature, treatment and prognosis of malignant lymphoma in head and neck.@*METHOD@#One hundred and sixteen cases with malignant lymphoma in head and neck were retrospectively analyzed. The predilection site was most in head and neck (39.7%), followed by palate and tonsil (23.3%). The clinical symptoms was different based on different location, painless mass in neck was mostly seen. The misdiagnosis rate is 37.1%. The treatment measures mainly included combined modality therapy and chemotherapy.@*RESULT@#Among 98 cases, of stage I and II, the survival time of 51 cases was more than one year, 24 cases were more than 3 years, 12 cases were more than 5 years. Eleven patients were lost to follow-up. Among 18 cases, of stage III and IV, 3 patients was lost to follow-up, the other 15 cases were dead after half a year.@*CONCLUSION@#clinical feature of malignant lymphoma in head and neck is nonspecific and complicated, misdiagnosis most easily occurs. Biopsy is the most valuable in the diagnosis of malignant lymphoma. Combined modality therapy is the mainstay of treatment in malignant lymphoma. The clinical stage classification is important for prognosis.

The study of mechanism for exendin-4(exenatide)improving insulin resistance / 中国糖尿病杂志

Objective To investigate the effects of exendin-4 (exenatide) on insulin sensitivity and adipocytokine in high-fat-fed rats. Methods Rats were divided randomly into normal-chow group (NC), high-fat group (HF) and high-fat+exendin treated group (HE). HE rats were given exenatide (2 μg/kg) twice daily for 6 wk. The insulin sensitivity was evaluated by intravenous insulin tolerance test (IVITT). Insulin-stimulated changes in insulin signal transduction, visfatin and adiponectin mRNA expressions as well as their plasma levels were also observed in these rats. Results Plasma free fatty acids (FFA), triglyceride (TG), total cholesterol (TC) levels were significantly reduced after exenatide treatment (in HE rats all P<0.01). And IVITT parameters were also improved in these rats. Insulin-stimulated IRS-1 tyrosine phosphorylation was slightly increased in exenatide-treated rats as compared with HF rats (P<0.05). In addition,plasma visfatin level was significantly reduced in HF and HE groups as compared with controls (P<0.05 and P<0.01). The adiponectin mRNA expression in adipose tissues and circulating adiponectin level were significantly elevated in exenatide-treated rats as compared with untreated rats and controls (P<0.01). Conclusions Chronic exenatide treatment improves insulin resistance in high-fat-fed rats, and the changes of IRS-1 tyrosine phosphorylation and adiponectin may be related to the role of exenatide in elevating insulin sensitivity

Relevance of Endoglin (CD105) VEGF and p53 with invasion metastasis and prognosis of laryngeal carcinoma / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the clinical significance of microvessel density (MVD) and investigate the relationship of Endoglin (CD105), VEGF and p53 protein and their significance of clinical pathology.@*METHOD@#Pathologic paraffin-embedded tissues and clinic data of 40 patients with primary squamous cell carcinoma of larynx were studied. Serial sections were respectively stained with Endoglin (CD105), VEGF and p53 by immunohistochemistry and its expressions were investigated. Microvessel density (MVD) highlighted by Endoglin (CD105) were counted according to a standard protocol.@*RESULT@#Endoglin (CD105) expression in tumour tissue was significantly higher than in normal mucosa (P < 0.05). The mean MVD value for Endoglin (CD105) was 14. 90 +/- 7.40. The mean CD105-MVD value of T3 and T4 tumours showed a significantly higher staining than that of T1 and T2 tumours; The mean CD105-MVD value of tumours with metastasis was also higher than that of tumours with no metastasis (P < 0.05); The expression of VEGF was observed in cytoplasm of tumour cell and its positive rate was 77.5% in laryngeal carcinoma, which was significantly correlated with TNM stage and pathological differentiation of laryngeal carcinoma (P < 0.05). The expression of p53 protein was mainly observed in nucleolus of tumour cell and its positive rate was 67.5%, which was significantly correlated with metastasis of lymph node. Positive relevance was found between CD105-MVD and VEGF (r = 0.641, P < 0.01); Positive relevance was also found between CD105-MVD and p53 (r = 0.534, P < 0.01).@*CONCLUSION@#Endoglin (CD105) is a marker of tumour angiogenesis for its significant associated with active angiogenesis in laryngeal carcinoma. The study shows that CD105-MVD in laryngeal carcinoma is an independent indicator of predicting invasion, metastasis and recurrence, and evaluating prognosis of malignant tumours. CD105-MVD, VEGF and p53 could be important indicators to evaluate invasion, metastasis and recurrence of laryngeal carcinoma.

Effects of Naohuandan Recipe on learning and memory abilities of SAM-P/8 mice and its role in anti-oxidation and anti-apoptosis / 中西医结合学报

OBJECTIVE: To investigate the effects of Naohuandan Recipe on learning and memory abilities of SAM-P/8 mice and its role in anti-oxidation and anti-apoptosis. METHODS: Forty SAM-P/8 mice were randomly divided into four groups, which were untreated (normal saline-treated) group, Yinkeluo Tablets (extracts of gingko leaf)-treated group, low-dose Naohuandan Recipe-treated group and high-dose Naohuandan Recipe-treated group. Mice in these groups were given corresponding drugs orally for 105 days. Then the performances of learning and memory of mice were tested by a step-down passive avoidance task and a Y-maze test. The serum levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were detected. The expression level of bcl-xl mRNA in cerebral cortex and hippocampus of mice was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The performances of learning and memory in the Yinkeluo Tablets-treated group, low- and high-dose Naohuandan Recipe-treated groups were significantly improved as compared with those in the untreated group (P<0.05 or P<0.01), and such performance was the best in the high-dose Naohuandan Recipe-treated group among these four groups (P<0.01). The serum levels of SOD and GSH-Px and the expression of bcl-xl mRNA in cerebral cortex and hippocampus of mice in the Yinkeluo Tablets-treated group, low- and high-dose Naohuandan Recipe-treated groups were also significantly higher than those in the untreated group (P<0.05 or P<0.01), while the serum level of MDA in the untreated group was higher than that in the other three groups (P<0.01). CONCLUSION: Naohuandan Recipe can improve learning and memory abilities of SAM-P/8 mice, and this effect may be related to its anti-oxidation efficacy and enhancement of expression level of bcl-xl mRNA.

Significance of CD105 expression in laryngeal carcinoma / 基础医学与临床

Objective To study the expression of CD105 in laryngeal carcinoma and to evaluate the clinical significance of microvessel density(MVD).Methods Pathologic paraffin-embedded sections and clinical data from 40 patients with primary squmamous cell carcinoma of larynx were studied through immunohistochemistry.Microvessel density(MVD) highlighted by CD105 and CD34 were counted.Results CD105 expression in tumour tissue was significantly higher than normal health mucosa.The mean CD105-MVD value of T3 and T4 tumours showed a significantly deeper staining than T1 and T2;The mean CD105-MVD value of tumours with metastasis was also markedly higher than tumours with no metastasis;The diffenerce of mean CD105-MVD value between in 16 patients with recurred laryngeal carcinoma and in 20 patients with unrecurred laryngeal carcinoma for two years of follow-up was markedly significant(P